KINETIC STUDIES ON IMMUNE HEMOLYSIS
نویسندگان
چکیده
منابع مشابه
Kinetic Studies on Immune Hemolysis
A quantitative method for the study of the kinetics of immune hemolysis is described. In the presence of excess C' and limited amounts of antibody the kinetics of hemolysis resemble those of enzymatic processes. Assays of hemolytic antibody should therefore be based on velocity measurements rather than end point titrations.
متن کاملStudies on Immune Human Hemolysis
The Donath-Landsteiner reaction was studied using low and high titer antisera and purified antibody, normal and PNH erythrocytes, and human serum complement. The requirement for complement in both the cold and warm phases of the reaction depended upon the level of antibody used and the sensitivity of the cells to hemolytic antibodies. Complement was not necessary in the cold phase using PNH cel...
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Additional experimental work on the subject of eosin hemolysis has been carried out. This indicates that red cells may be protected against the toxic action of eosin in sunlight by the presence of inorganic reducing agents. It is pointed out that a marked parallelism exists between the substances which react with the Folin and Denis reagent and the compounds which afford protection to red cells...
متن کاملNon-immune Hemolysis: Diagnostic Considerations.
Non-immune hemolytic anemia (NIHA) is characterized by positive routine hemolytic tests but negative anti-human immunoglobulin (Coombs) test. Hereditary non-immune hemolysis includes disorders of erythrocytic enzymes, membrane, hemoglobin (qualitative and quantitative disorders), as well as the rare hereditary forms of thrombotic microangiopathies. Acquired NIHA includes paroxysmal nocturnal he...
متن کاملStudies on Immune Human Hemolysis I. the Kinetics of the Donath-landsteiner Reaction
Serum complement is of potential importance to clinical medicine and to human biology in immune reactions and hypersensitivity states. However, most studies on the characteristics and mechanism of action of complement have been performed on systems largely of non-human origin (1, 2). The present studies were undertaken to study a specific immune human hemolytic disorder and to use this as a mod...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1948
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.88.4.427